Determining the Kd of a Molecule Against PPT1
Microscale thermophoresis was used to test the compound's binding to recombinant human PPT1, alongside control inhibitors.
Challenge
The client wanted to know whether their molecule bound to PPT1 and whether a similar molecule also bound at the same site.
What We Did
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1
Tested binding with microscale thermophoresis
Assessed the compound's binding to recombinant human PPT1, alongside control inhibitors.
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2
Reported the experimental work
Prepared a full report for the client covering the methods and results.