Determining the Kd of a Molecule Against PPT1

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Case Study 07

Determining the Kd of a Molecule Against PPT1

Microscale thermophoresis was used to test the compound's binding to recombinant human PPT1, alongside control inhibitors.

  • PPT1
  • Binding assessment
  • Microscale thermophoresis
  • Control inhibitors

Challenge

The client wanted to know whether their molecule bound to PPT1 and whether a similar molecule also bound at the same site.

What We Did

  1. 1
    Tested binding with microscale thermophoresis

    Assessed the compound's binding to recombinant human PPT1, alongside control inhibitors.

  2. 2
    Reported the experimental work

    Prepared a publication quality report with detailed methods and results.

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Identifying Dual Kinase Inhibitors

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Designing New Polymers for Human Health Applications